Evaluation of the Antioxidant, Anti-Proliferative and Anti-Seizure Effects of Ethanol Leaf Extract of Strychnos innocua Del.
Chinedu Enegide*1, Divine Odeworitse1, Israel Ofejiro Efejene1,3, Nwabenu Opia Okwaji2, Quincy Asheneyinoritse Ogio1, Eugene Ehimare1 and Esther Adaeze Ossai1
- Department of Pharmacology, College of Medical & Health Sciences, Novena University, Ogume, Delta State, Nigeria
- Department of Pharmacology & Toxicology, Faculty of Pharmacy, Novena University, Ogume, Delta State, Nigeria
- Department of Pharmacology, Faculty of Basic Medical Sciences, Southern Delta University,
Ozoro, Delta State, Nigeria
| Key words:
Antioxidant, antiproliferative, antiseizure, Strychnos innocua, ABTS
*Corresponding author: chinex.snow@gmail.com; Page No: 136-144 |
AbstractStrychnos innocua is a small tree belonging to the Loganiaceae family. In ethnomedicine, it has been used to treat bacterial infections, central nervous system disorders, and other ailments, with anecdotal claims of anticancer efficacy. This study aimed to evaluate the antioxidant, antiproliferative, and anti-seizure activities of an ethanolic leaf extract of S. innocua. The antioxidant effect was evaluated using 2,2’-azino-bis (3ethylbenzthiazoline-6sulphonic acid) (ABTS) radical scavenging activity, total flavonoid, total phenolic, ferric reducing antioxidant power, and total antioxidant capacity assays. The effects of the rapidly proliferating seed radicles were evaluated to determine their antiproliferative potential. The LD50 was evaluated in mice using Lorke’s method, while the isoniazid-induced seizure mouse model was used to assess the anti-seizure effect. The results revealed that the extract demonstrated good antioxidant activity, with 55.69% inhibition at 400 μg/mL in the ABTS assay. The extract also showed significant (P < 0.05) antiproliferative activity at 10 – 50 mg/mL. The extract LD50 was greater than 5000 mg/kg p. o. and demonstrated a significant (P < 0.05) antiseizure effect at all doses (250 – 1000 mg/kg).
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