Anti-arthritic and Antioxidant Potentials of Leaf Extract of Atrocarpus heterophyllus Lam 

Anti-arthritic and Antioxidant Potentials of Leaf Extract of Atrocarpus heterophyllus Lam


Victoria Nonyelum Olli1* Nneoma Ngozi Okpalanma2, Ernest C. Orji3, Jude Nnaemeka Okoyeh4

1Department of Pharmacology and Toxicology, Chukwuemeka Odumegwu Ojukwu University

Igbariam Campus, Nigeria,

2Department of Clinical and Pharmacy Management, Chukwuemeka Odumegwu Ojukwu University Igbariam Campus, Nigeria,

3Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, P.M.B 5025 Awka,420110 Anambra state, Nigeria

4Department of Medical Laboratory Science and Biology, School of Nursing and Health Sciences, Neumann University, One Neumann Drive, Aston, PA, 19014. USA


Keywords; Artocarpus heterophyllus, leaf extract, anti-arthritis, anti-oxidant.





*Corresponding author:,


Page No: 91-100
Volume:5, Issue 2, 2024
Trends in Natural Products Research
Copy Right: NAPREG


Oxidative stress predisposes human and animal bodies to diseases like cancer, diabetes, rheumatoid arthritis, atherosclerosis and chronic inflammatory disorders. This study is aimed to determine the antioxidant, and anti-arthritic activities of ethanol leaf extract of Atrocarpus heterophyllus   which have been used traditionally to treat arthritis and inflammation. The shade-dried and pulverized leaves of A. heterophyllus were cold macerated in 80 % ethanol for 48 hours and filtered; the filtrate was dried over water bath at 40 ºC. Acute toxicity study and phyto chemical screening were carried out.  The in-vivo anti-arthritic property of the extract was tested orally on formalin induced arthritic model at 250 and 500mg/kg doses for 8 days using a set of 20 albino rats of both sexes that were grouped into four (n=5). Groups I and II served as the negative (10ml/kg distilled water) and positive (diclofenac sodium 10mg/kg) controls, respectively. Groups III and IV received 250 and 500 mg/kg of the extract, respectively. In-vitro antioxidant activities of the extract were determined at 517 nm with spectrophotometer using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) model. Histopathological examinations of the proximal interphalangeal joints from the induced arthritic rats were also carried out. The estimated oral median lethal dose (LD50) of the extract was higher than 5000 mg/kg. Secondary metabolites such as tannins and saponins were present in the extract. The extract conferred a dose-related significant (p<0.05) reduction in paw diameter when compared to control. The in-vitro IC50 values for the extract (9.62ug/ml) were higher than that of standard drug vitamin C (6.12ug/ml). The result of the histopathology analysis revealed a dose-dependent anti-arthritic potential of the leaf extract from Atrocarpus heterophyllu