Antibacterial activities of four medicated soaps on clinical bacterial isolates

Antibacterial activities of four medicated soaps on clinical bacterial isolates

Enemuor Simeon Chukwuemeka*, Ugwuanyi Joshua Somtochukwu, Ezeh Christian Kelechi

Biocatalysis and Environmental Health Research Group

Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.


Key words:

Medicated soap, Antibacterial activity, Active antimicrobial ingredient.





*Corresponding author:,

DOI: 10.48245/tnpr-2734391.2022.3.101

Page No: 18-28
Volume: 4, Issue 1, 2023
Trends in Natural Products Research
Copy Right: NAPREG


Medicated soaps contain antibacterial properties to care for the skin. This study was aimed at evaluating the antibacterial activities of four medicated soaps; Sample A, B, C and D against clinical bacterial isolates namely: Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. Antibacterial activity was carried out using both Agar disk and Agar-well diffusion techniques at concentrations of 200, 100, 50, 20, 10 mg/ml on Mueller-Hinton medium and distilled water was used as a negative control. The experiments were carried out in duplicates and the zones of inhibition recorded in millimeters. Sample A which contained Chloroxylenol (0.5 %) inhibited the growth of Bacillus subtilis, S. aureus and E. coli. Sample B which contained Monosulfiram (5 %) showed highest inhibitory activity against E. coli, while Sample C containing Triclosan (0.5 %) as active ingredient showed the highest antibacterial activity against P. aeruginosa at the lowest concentration used. Sample D containing Chloroxylenol (0.3 %) showed low inhibition zones compared to other soap samples and this could be due to the low concentration of the active ingredient. The Minimum inhibitory concentrations (MIC) of Sample B and C was 20 mg/ml against P. aeruginosa and E. coli, while that of Sample A was 50 mg/ml for B. subtilis and S. aureus. The minimum bactericidal concentration (MBC) Sample B and C for P. aeruginosa and E. coli was 50 mg/ml and MBC of Sample A for B. subtilis and S. aureus was 100 mg/ml. From our findings it was obvious that Sample A displayed high activity at low concentrations and therefore, can be considered as good antiseptic soaps for topical cleansing purposes. However, the concentrations of the active ingredients (Chloroxylenol) which are known to have broad spectrum activity should be properly monitored in products prepared for topical use to avoid counter effects on the users.