Antihyperlipidemic and Antioxidant Activities of Catechin-rich Extract and Fractions of Phaseolus vulgaris, A Traditional Therapy for Overweight Diabetes Subjects


Antihyperlipidemic and Antioxidant Activities of Catechin-rich Extract and Fractions of Phaseolus vulgaris, A Traditional Therapy for Overweight Diabetes Subjects

Ejiroghene Ahante1*, Itohan Mercy Osifo1, Daniel Lotanna Ajaghaku2, Moke Emuesiri Goodies3, Festus Basden Chiedu Okoye4, Chukwuemeka Sylvester Nworu1

  1. Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
  2. Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, Enugu State University, Enugu State, Nigeria
  3. Department of Pharmacology and Therapeutics, Delta State University, Abraka, Delta State, Nigeria
  4. Department of Pharmaceutical & Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

 

Key words: Catechin, Phaseolus vulgaris,   hyperlipidemia, antioxidant

 

 

 

 

*Corresponding author:

ejiroahante@gmail.com;

+234 8160021409

 

 

 

 

 

Page No.: 20-33
Volume: 1, Issue 1, 2020
Trends in Natural Products Research
Copy Right: NAPREG

Abstract

Phaseolus vulgaris is used in ethnomedicine for the management of overweight and cardiovascular disorders with claims of efficacy. This motivated this study in which the antihyperlipidemic activities of the extracts of P. vulgaris and its antioxidant activities were evaluated. Methanol/methylene chloride (1:1) extract was screened and further partitioned into n-hexane, ethyl acetate and butanol solvent fractions. HPLC “fingerprinting” technique showed the abundance of catechin in the crude extract. Oral median lethal dose (LD50) was estimated to be greater than 5000 mg/kg. Short term treatment with the crude extract and ethyl acetate fraction significantly (p < 0.05) lowered the levels of serum triglyceride, total cholesterol, LDL-C and VLDL-C in hyperlipidemic condition induced in rats with Triton WR-1339, Triton X-100 and high fat diet. Similarly, sub-acute treatment of the animals with crude extract and ethyl acetate fractions produced dose-dependent and significant (p < 0.05) decrease in serum triglyceride, total cholesterol and VLDL-C levels in high fat hyperlipidemic model. Antioxidant activities of the extract and fractions were determined in vitro using DPPH assay while liver malondialdehyde and serum antioxidant enzyme activities were used to assess the in vivo antioxidant potentials in CCl4-intoxicated rats. DPPH median inhibitory concentrations (IC50) of the extract and fractions ranged from 79.84-98.49 μg/ml. The extract and its fractions produced significant (p < 0.05) inhibition of lipid peroxidation and significant (p < 0.05) higher levels of catalase and glutathione peroxidase enzyme activities. This investigation showed that the extract and fractions of Phaseolus vulgaris possess some anti-hyperlipidemic and antioxidant activities that are relevant to its use in traditional medicine.