Comparison of the Antiulcer Effects of the Fractions of Ethanol Extracts of Musa sapientum Linn and Brassica oleracea Linn in Albino Rats
Sussan Chineta Obianuo 1,2*, Prince Chiazor Unekwe1, and Ejeaturuchukwu Obi1.
1.Department of Pharmacology and Therapeutics, College of Medicine, Nnamdi Azikiwe University, Awka, Anambra State.
2.Department of Pharmacology, Benjamin Carson Senior School of Medicine, Babcock University, Ilisan-Remo, Ogun State.
| Key words:
Musa sapientum, Brassica oleracea, ulceration, cytoprotection
*Corresponding author: 08063771880 DOI: 10.48245/tnpr-2734391.2021.1.205 Page No.: 109-116 |
Abstract The development of new anti-ulcer drugs from medicinal plants is an attractive field. Diverse chemical compounds with anti-ulcer activities have been isolated from plants. This study compared the antiulcer potentials of the fractions of ethanol extracts Musa sapientum unripe fruits and Brassica oleracea leaves in albino rats. Extraction was by cold maceration in ethanol to obtain the ethanol extracts of both plants. The ethanol extracts were fractionated by liquid-liquid partitioning method using n-hexane, chloroform, n-butanol and water to obtain the various fractions; HFm, CFm, BFm, and WFm of M.sapientum and HFb, CFb, BFb, and WFb of B.oleracea respectively. The oral acute toxicity (LD50) of the extracts was determined in mice. Pilot study was carried out to determine the fraction with highest anti-ulcer effects which was then used for the comparative study. Doses of 100 mg/kg of WFm and WFb and standard dose of misoprostol were administered to three different groups of albino rats for 21 days after which ulcer was induced with 1ml of 98 % ethanol. One hour after ulcer induction, the animals were sacrificed under diethyl ether anaesthesia and, their stomachs were harvested and opened for ulcer ratings. The LD50 of the extracts were more than 5000 mg/kg. The ulcer index was decreased from 6.68 to 1.87, 2.97 and 3.85 by WFm, WFb and misoprostol respectively. The percentage ulcer protection was 72 %, 55.5 % and 12.4 % for WFm, WFb and misoprostol respectively. The results indicate that the unripe fruit of M. sapientum exhibited better ulcer protection than the leaves of B. oleracea
|