Evaluation of the Anti-Inflammatory and Antioxidant, Activities of Parinari Curatellifolia Planch. Ex Benth. (Chrysobalanaceae) Methanol Stem Bark Extract


Evaluation of the Anti-Inflammatory and Antioxidant, Activities of Parinari Curatellifolia Planch. Ex Benth. (Chrysobalanaceae) Methanol Stem Bark Extract

Theodora Chidinma2, Michael Onyegbulam Chukwuma1*, Estella Uchenna Odoh1, Ikenna Chikeokwu2

  1. Department of Pharmacognosy and Environmental Medicine, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nsukka 410001, Nigeria.
  2. Department of Pharmacognosy, Enugu State University of Science and Technology, PMB 01660, Agbani, 401150, Enugu state, Nigeria

 
 

Keywords;

Phoenix reclinata, methanol extract, anti-inflammation, antipyretic

 

 

 

 

*Corresponding author: chukwuma.michael@unn.edu.ng.

DOI:https://doi.org/10.61594/tnpr.v5i1.2024.105 

Page No: 52-62
Volume:5, Issue 1, 2024
Trends in Natural Products Research
Copy Right: NAPREG

           Abstract

It is used as human food and beverage, and in traditional medicine it is used to treat pneumonia and fever. The aim of this was to investigate its anti-inflammatory and antioxidant activities of Parinari curatellifolia methanol stem bark extract. A 2kg of the dried and powdered plant material was extracted with 80% methanol through cold maceration to obtain the crude methanol extract. The crude methanol extract was sequentially fractionated using n-hexane, ethyl acetate and methanol to yield n-hexane, ethyl acetate and methanol fractions respectively. Acute toxicity study was conducted following the OECD guidelines. The anti-inflammatory activity of the fractions was evaluated was evaluated using inhibition of albumin denaturation, inhibition of egg albumin-induced paw edema in rats and phospholipase A2 (PLA2) inhibition assay models. In vitro antioxidant activity was evaluated using DPPH de-colourizing, Nitric oxide scavenging activity and Ferric Reducing Antioxidant Power (FRAP) assays. Oral acute administration of the extract revealed an LD50 dose > 5000 mg/kg in rats. The extract caused a dose- and time-dependent inhibition of rat paw edema, and a concentration-dependent inhibition of Phospholipase A2 activity. IC50 of the extract for Albumin Denaturation inhibition was 37.22 ug/ml. In the antioxidant assays the IC50 of the extract against DPPH was 25.91 ug/ml, while the Nitric oxide Equivalence (mM)/mg was 42.38.