Fractions of Monodora myristica (Gaertn.) Dunal (Annonaceae) root bark possesses anti-inflammatory activity


Fractions of Monodora myristica (Gaertn.) Dunal (Annonaceae) root bark possesses anti-inflammatory activity.

Isiogugu Ogechukwu Nnanyelugo1*, Peter Ikechukwu Emmanuel1, Ofokansi Martha Nneoma1, Alozie Eleazar Michael1, Abonyi Uchenna Collins1, Ogbue Cyril Onyeka2, Ezike Adaobi Chioma1

  1. Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria.
  2. Department of Pharmacognosy and Traditional Medicine, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

 
 

Keywords; Carrageenan, formaldehyde, granuloma, Monodora myristica, xylene

 

 

 

 

*Corresponding author: ogechukwu.isiogugu@unn.edu.ng

DOI: 10.48245/tnpr-2734391.2023.4.203

Page No: 61-70
Volume:4, Issue 2, 2023
Trends in Natural Products Research
Copy Right: NAPREG

Abstract

This evaluated the acute and chronic anti-inflammatory activity of Monodora myristica root bark extract and fractions in rodents. The methanol crude extract (CE) as well as the hexane (HF), ethyl acetate (EAF) and methanol (MF) fractions were prepared and screened for anti-inflammatory activity employing carrageenan induced rat paw edema, xylene induced topical ear edema, formaldehyde induced arthritis (FIA) and the cotton pellet induced granuloma (CPIG) models. Phytochemical screening as well as acute toxicity (LD50) tests were equally evaluated. The EAF (400 mg/kg and 800 mg/kg) and MF (200 mg/kg) elicited significant (p < 0.05) and time-course inhibition of carrageenan induced rat paw edema. All the fractions exhibited significant (p < 0.05) inhibition of xylene induced topical ear edema. The extract and fractions evoked moderate inhibition of formaldehyde induced arthritis and the cotton pellet induced granuloma. The fractions gave positive reaction for alkaloids, glycosides, saponins, flavonoids, tannin, acid compounds, resins, steroids, terpenoids, protein, reducing sugars, carbohydrates, fats and oil in varying degrees. The acute toxicity screening showed no mortality at 5000 mg/kg of the extract and fractions after 48 hours of observation period. The results suggests that the root bark of M. myristica possesses anti-inflammatory effect which corroborates its folkloric use in managing edematous conditions.