GC-MS Profile and protective effect of Abelmoschus esculentus L, Moench, leaf on monosodium glutamate-induced organ pathologies.
Temitayo Esther AdeyeOluwa1*, Tofunmi Danielle Olagundoye2, Olanrewaju Olaifa3 Roseangela Ifeyinwa Nwuba2
- Department of Pharmacology and Therapeutics, University of Medical Sciences, Ondo.
- Department of Bioscience and Biotechnology, University of Medical Sciences, Ondo.
- Department of Pathology, University of Ibadan, Ibadan.
| Key words:
Abelmoschus esculentus, Monosodium glutamate, Wistar rats, Gas Chromatography-Mass Spectrometry (GC-MS)
*Corresponding author: tadeyeoluwa@unimed.edu.ng; Page No: 289-302 |
AbstractAbelmoschus esculentus is a nutritional herb that is consumed in some cultures in Africa and Asia. Its leaf extract has been demonstrated to possess optimal antioxidant activity. This study evaluated the Gas Chromatography-Mass Spectrometry (GC-MS) profile of the ethanol extract of Abelmoschus esculentus leaf as well as its protective effect on Monosodium glutamate (MSG)-induced vital organs toxicity. The ethanol extract of Abelmoschus esculentus leaf (EAEL) was profiled for its compounds by the GC-MS method using the Agilent 5977B A GC with Agilent 8860 with an Elite-5MS column (30 m x 0.320 mm i. d. x 0.25 μm df). The effects of the extract (76.92 mg/kg; p.o) on liver, kidney, heart and uterine histology as well as functions were evaluated using female. The GC-MS revealed the presence of several compounds with 9-Octadecenoic acid, Hexadecanoic acid methyl ester, Methyl stearate, 9,12,15-Octadecatrienoic acid and Pentadecanoic acid being prominent. EAEL reversed some of the MSG-induced histological anomalies in the 4 tissues and ameliorated the biochemical parameters affected by MSG treatment. This study reveals the active compounds in the ethanol extract of Abelmoschus esculentus leaf as well as their potentials in reversing MSG-induced organ anomalies.
|