In Vitro Anti-Diabetic and Antioxidant activities of ethanol leaf extract of the Nephrolepis Exaltata (L.) SCHOTT. (Nephrolepidaceae)

In vitro anti-diabetic and antioxidant activities of ethanol leaf extract of the Nephrolepis exaltata (l.) Schott. (Nephrolepidaceae).

Aminat Asabi Oyawaluja1* Joseph Oisemuzeimen Oiseoghaede1, Nurat Bisola Bello1, Titilola
Aderonke Samuel2, and Olukemi Abiodun Odukoya1

  1. Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.
  2. Department of Biochemistry, University of Lagos, Nigeria.


Keywords; Nephrploepis exaltata, α –amylase, α – glucosidase, antioxidant, Diabetes.





*Corresponding author:

DOI: 10.48245/tnpr-2734391.2023.4.201

Page No: 45-51
Volume:4, Issue 2, 2023
Trends in Natural Products Research
Copy Right: NAPREG


Diabetes is one of the non-communicable diseases growing rapidly worldwide with increasing prevalence in children adolescence and young adults. The study of alpha–amylase and alpha-glucosidase inhibitory activities of agents provides an insight of their possible antidiabetic properties. The enzymes α–amylase and α-glucosidase are responsible for postprandial glucose levels. The aim of this study was to investigate the antioxidant and inhibitory potentials of the ethanol leaf extract of Nephrolepis exaltata (L.) Schott. (Nephrolepidaceae) against α–amylase and α-glucosidase in vitro. DPPH (1,1 diphenyl-2-picryl hydrazyl) free radical scavenging activity and lipid peroxidation assay were used to assess the antioxidant assay of N. exaltata using ascorbic acid as standard. Alpha-amylase and alpha-glucosidase inhibitory activities were evaluated. Preliminary phytochemical screening showed the presence of phenols, tannins, flavonoids, alkaloids, resins, saponins and carbohydrate. The inhibitory concentration (IC50) for DPPH assay was 5.83 μg/ml and 0.04 μg/ml for anti-lipid peroxidation assay were obtained for the radical scavenging activities, while IC50 of 0.05 μg/ml and 0.03 μg/ml were obtained for the α-amylase and α-glucosidase inhibitory assay respectively. The ethanol leaf extract of Nephrolepis exaltata possesses antioxidant activities useful and inhibited α-amylase and α–glucosidase which are responsible for postprandial glucose levels.