Influence of Glycerogelatin on the Antibacterial Activity of Limosilactobacillus reuteri CF48-3A Against Escherichia coli.
Enosakhare Oloton* and Amomatorione Excellent Yaluwu.
- Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
| Key words:
Escherichia coli ATCC 25922, Glycerogelatin, Limosilactobacillus reuteri C3A, Probiotics antagonism, Probiotic pessaries.
*Corresponding Author: enosakhare.oloton@uniben.edu
Page No: 307-313
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AbstractEscherichia coli is one of the most frequently encountered bacteria in aerobic vaginitis. This study evaluated the influence of glycerogelatin base on the antibacterial activity of Limosilactobacillus reuteri CF48-3A against Escherichia coli ATCC 25922. Both strains were characterized using phenotypic and biochemical methods. Antibacterial activities were assessed using the agar overlay method, while co-cultivation assessed comparative E. coli growth inhibitory patterns in combination with L. reuteri CF48-3A and glycerogelatin base. Statistical significance was determined using two-factor ANOVA. Characterization confirmed the identity of both organisms. The agar overlay method demonstrated only bacteriostatic activity (IZD = 30.5 ± 0.44). Co-cultivation reduced E. coli growth to 0.38 log₁₀ CFU/mL/h (21.4%) in the presence of L. reuteri CF48-3A compared with control, indicating antagonism. An addition of glycerogelatin to the system further reduced to 0.18 log₁₀ CFU/mL/h (56.1%). This reduction was enhanced with the presence of glycerogelatin base. Glycerogelatin produced a highly significant antibacterial activity (Two way ANOVA), with Turkey’s post hoc analysis showing significant differences (P < 0.05) compared to other groups. L. reuteri CF48-3A is a promising probiotic candidate for inhibiting E. coli ATCC 25922, while, glycerogelatin base is an effective base for optimizing its antibacterial activity.
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